| ABSTRACT: |
|
Ellagic acid (EA) was fed to trout fry during or following DMBA
initiation (5 hr immersion in 5 ppm DMBA on day 7) in a 9 mo tumor study.
2000 ppm EA fed during initiation (days 1-14) reduced stomach
adenopapilloma multiplicity (4.2 vs 7.8) and incidence (78% vs 88%,
p=0.023), with near significance in gas bladder (7% vs 15%, p=0.06).
Groups fed 0, 1000, or 2000 ppm EA for wk 4-21 after DMBA treatment had
88%, 26%, and 19% stomach (p less than 0.001), and 15%, 6%, and 5.6% gas
bladder incidence (p less than 0.05), resp. Since continuous EA suppressed
average growth rate (52-55% of control), stomach data were analyzed among
EA and control groups with similar growth. Incidence increased linearly
with mean growth rate up to the 80 g class, and plateaued at 96% incidence
in control, 69% incidence EA above this weight. In all growth classes, EA
groups had lower incidence (mean difference 28%). Thus EA effectively
suppressed tumors independently of growth inhibition. An ongoing
confirmatory EA study without growth inhibition will be discussed. |